CONTENTS
 
Introduction to the Series  xi
1. Introduction  1
2. Photobiological Nature of Laser Biostimulation Effects  5
    2.1 Role of light coherence  5
    2.2 Historical review 
3. The Action of Monochromatic Visible Light at Cellular Level  11
    3.1 Enhancement of multiplication of microorganisms  11
    3.2 Effects of irradiation on proliferation of mammalian cellular  
          cultures 
27
          3.2.1 Viability and clonigenicity of plateau phase HeLa cells after  
                   irradiation  with He-Ne laser 
30 
    3.3 Changes in DNA and RNA synthesis rate after the irradiation  36
          3.3.1 Exponentially growing HeLa cells  38 
                   a) Effect of cw light  38
                   b) Effect of pulsed light  39 
                   c) Role of light intensity  46 
                   d) Duration of stimulation  49 
                   e) Action spectra in whole visible region  49 
          3.3.2 Plateau phase HeLa cells  53
          3.3.3 Disappearance of the effects by widening the   
                   light band 
55 
    3.4 Changes in cell cycle parameters after the irradiation  57
    3.5 Human lymphocyte chromatin changes following   
          irradiation with He-Ne laser 
62
          3.5.1 Conformational changes  63
          3.5.2 Blasttransformation  67
    3.6 Effect of visible light on chromosomal abnormalities  
          in mammalian cells 
71
    3.7 He-Ne laser radiation as antimutagenic and  
          radioprotective factor 
73
    3.8 Changes in activity of enzymes of oxidative and phosphorous  
          metabolism after irradiation of cells with He-Ne laser 
77
4. Short-Term Responses to Irradiation  85
    4.1 Stimulation of mitochondrial functions  85 
    4.2 Responses connected with cellular membrane  87
          4.2.1 Changes in cAMP level of Chinese hamster  
           fibroblasts 
88
    4.3 Is it possible to cause the changes in cellular membrane  
          parameters when irradiating mitochondria? 
  
92
5. Primary Photoacceptors  95
    5.1 Action spectra of photoresponses and absorption  
          spectra of respiratory chains 
95
    5.2 Influence of dichromatic irradiation on the final  
          photoresponse 
97
          5.2.1 Action of red and far red light upon HeLa cells  102
          5.2.2 Action of red and far red light upon E. coli  106 
          5.2.3 Action of blue and red light upon HeLa cells  108
          5.2.4 Action of blue and red light upon E. coli  111 
    5.3 Modification of light responses with chemicals  111 
    5.4 Connection of light responses with the temporal  
          organization of the respiratory chain 
122 
    5.5 Are the same primary photoacceptors responsible  
          for stimulatory and inhibitory action of light? 
123
6. Mechanism of Light Action on Cellular Level  131
    6.1 Signal transduction chains in prokaryotic cells  131 
    6.2 Signal transduction chains in eukaryotic cells  133 
    6.3 Limits of photostimulation effects magnitude  138
    6.4 Possible connection of light-stimulated effects with  
          existing cellular periodicity's 
143
    6.5 Summary of the experiments performed at cellular level  146 
7. The Action of Monochromatic Visible Light at  
    Organism Level 
151 
    7.1 Penetration of light into tissues  151
    7.2 Animal experiments  153
    7.3 Low-power laser therapy in clinical practice 155 
          7.3.1 Phototherapy of gastric and duodenal ulcers 156
    7.4 On the mechanism of low-power laser therapy 161
References  168 
Abbreviations  185
Subject Index  187